Punisher: War Zone is an action film primarily focused on Frank Castle's vigilante justice. The movie does not include any discernible LGBTQ+ characters or themes, resulting in no specific portrayal to evaluate.

Based on available information and the film's known plot, there are no identifiable transsexual characters or themes present in "Punisher: War Zone." The narrative focuses on Frank Castle's vigilante actions against organized crime, without incorporating any elements related to transgender identity.

The film does not feature any significant female characters engaging in direct physical combat. Female characters present are in non-combatant roles, primarily as victims or supporting figures, and do not defeat male opponents in close-quarters fights.

All major characters in Punisher: War Zone, including Frank Castle (Punisher), Jigsaw, and Microchip, maintain their established genders from the Marvel Comics source material. No significant character's gender was altered for this adaptation.

The primary characters, including Frank Castle (Punisher) and Billy Russoti (Jigsaw), maintain their established racial identities from the Marvel Comics source material. No canonical characters were portrayed by actors of a different race.