Queens of the Dead offers a highly affirming portrayal of the LGBTQ+ community, centering queer characters as vibrant, resilient, and heroic protagonists in a zombie apocalypse. The film celebrates queer culture, friendship, and solidarity with humor and camp, deliberately avoiding negative tropes and instead highlighting the community's strength and fighting spirit.

The film "Queens of the Dead" offers a primarily positive portrayal of transsexual and gender-nonconforming characters and themes. It features non-binary main characters and explicitly addresses pronoun usage, while centering gender expression and drag performance. The narrative empowers queer characters, positioning their unique identities and skills as vital for leadership and survival in a post-apocalyptic setting. For example, the film suggests that the end of the world unleashes leadership qualities in those who had a rough time existing in the old one.