Rebel Moon - Part One: A Child of Fire attempts LGBTQ+ inclusion with a nonbinary character. However, this is significantly overshadowed by a scene depicting a predatory gay alien who sexually assaults a hero, perpetuating a harmful homophobic trope. Despite efforts for representation, the problematic portrayal results in a net negative impact on queer themes.

Despite the inclusion of a nonbinary character, Milius, for diversity, the film's overall portrayal of transsexual and broader LGBTQ+ themes is negative. This is primarily due to a problematic scene perpetuating a harmful homophobic trope, which significantly overshadows the limited and underexplored positive representation.

The film features multiple female characters who engage in and win close-quarters physical combat against male opponents. Kora and Nemesis, in particular, demonstrate significant skill and strength in defeating groups of male soldiers using melee weapons and martial arts.

The film incorporates Christian motifs, particularly themes of personal and spiritual sacrifice, to enrich its mythic and epic tone. These elements are utilized respectfully to add narrative depth rather than being critiqued.