Scary Movie's portrayal of LGBTQ+ themes is predominantly negative, using queer identity as a source of problematic comedic shock. The film relies heavily on harmful stereotypes, gay panic, and homophobic slurs for humor, particularly through the character of Ray. There is no positive counterbalance, resulting in a depiction that mocks and degrades rather than affirms.

Scary Movie features multiple instances where characters' gender or sex characteristics are used for shock humor and ridicule. Jokes about characters having penises or being perceived as transsexual are consistently played for comedic disgust and discomfort, contributing to a problematic portrayal that lacks any affirming elements.

Several characters in "Scary Movie" are parodies of characters from "Scream" who were originally portrayed by white actors. For instance, the parodies of Stu Macher and Tatum Riley are played by Black actors Shawn Wayans and Regina Hall, respectively, constituting race swaps.

The film satirizes Christianity through the character of Father McFeely, portraying him as a lecherous and inappropriate priest. This depiction highlights hypocrisy and moral failings within religious institutions for comedic effect, without offering any counterbalancing positive or nuanced perspectives.