Seed of Chucky features Glen/Glenda, a central genderfluid character whose struggle with identity is a key narrative element. While one parent expresses prejudice, the other is accepting, and the film ultimately affirms the character's unique identity with complexity and agency, leading to a net positive portrayal.

Seed of Chucky features Glen/Glenda, a character exploring gender identity. The film portrays a supportive parent (Tiffany) who affirms Glenda's identity against the transphobic views of Chucky, the villain. Despite its comedic horror tone, the narrative ultimately allows for the expression and affirmation of Glen/Glenda's gender identities, leading to a net positive portrayal.

The film portrays a devout Christian nanny as overly strict, judgmental, and naive. Her faith is presented as a source of her rigid morality, which is satirized and ultimately does not protect her from the film's antagonists. The narrative offers no counterbalancing positive portrayal, reinforcing a negative stereotype.