The film "Shaft in Africa" does not feature any identifiable LGBTQ+ characters or themes within its narrative. The story focuses on John Shaft's mission to dismantle a human trafficking operation, with no elements related to queer identity or experiences.

Shaft in Africa is an action-adventure film that does not include any discernible transsexual characters or explore themes related to transgender identity. The plot centers on John Shaft's mission, with no elements pertaining to transsexual portrayal.

The film features female characters, including a skilled antagonist. However, no female character is depicted as defeating one or more male opponents in direct physical combat using skill, strength, or martial arts. The primary female antagonist engages in a fight with the protagonist but is ultimately defeated.

The film features John Shaft, who retains his established male gender. All other significant characters are new to this installment and do not have prior canonical or historical gender baselines that are altered.

The film continues the story of John Shaft, who was established as a Black character in previous installments and source material. He is consistently portrayed by a Black actor, Richard Roundtree, in this film. No other established characters undergo a race change.