Soul Plane features openly gay characters whose portrayals primarily rely on exaggerated, stereotypical mannerisms for comedic effect. Their identity is largely a source of mockery, with humor often derived from harmful stereotypes rather than offering any complex, dignified, or affirming representation of LGBTQ+ individuals or themes.

Soul Plane features a character whose trans identity is revealed as a comedic plot twist, eliciting shock and disgust from other characters. The portrayal uses trans identity as a source of mockery and reinforces harmful stereotypes, lacking dignity or empathy for the character.

The film features Reverend Hightower as a satirical, flamboyant, and somewhat hypocritical preacher, presenting a negative caricature of a religious leader without significant counterbalancing positive portrayals of the faith.

The portrayal of the Hunkee family relies heavily on Jewish stereotypes for comedic effect, reinforcing these stereotypes rather than offering nuance or challenging them.