The film 'Strawberry Shortcake: Berry Blossom Festival' does not include any identifiable LGBTQ+ characters or themes. The narrative centers on the main character and her friends in a G-rated, family-friendly context, with no elements related to queer identity present.

This children's animated film, part of the Strawberry Shortcake franchise, focuses on themes of friendship and adventure. It does not feature any identifiable transsexual characters or explore related themes, aligning with its target audience and genre.

The movie does not contain any action or adventure elements.

The film features established characters from the Strawberry Shortcake franchise, such as Strawberry Shortcake, Huckleberry Pie, and the Purple Pieman. All characters maintain their canonical genders from previous iterations and source material, with no instances of a character being portrayed as a different gender.

The film maintains the established racial depictions of its characters from the Strawberry Shortcake franchise, including Orange Blossom as Black and other core characters as white. No character originally established as one race is portrayed as a different race.