Sunshine Cleaning features a subplot involving a deceased trans woman, Lynn, and her grieving partner, Carl. The film portrays their relationship and Carl's loss with empathy and respect, affirming the worth of Lynn's life and their love through Carl's sincere mourning and the protagonists' evolving understanding. The depiction is largely affirming.

Sunshine Cleaning features Norah, a transsexual woman, as a loving and stable partner. While Rose's father initially struggles with Norah's identity, the narrative frames his prejudice as a flaw he overcomes, ultimately accepting and defending her. The film portrays Norah with dignity and empathy, affirming her worth and relationship, resulting in a net positive depiction.