Birdo, embraced by the LGBTQ+ community as a trans icon since her 1988 debut, features in a minor action role without the film addressing queer themes explicitly. This incidental inclusion neither uplifts nor denigrates LGBTQ+ identities, fitting neutrally into the family adventure.

Birdo, embraced by fans as a trans icon, features in a feminine role amid the galactic adventure, accepted seamlessly by the cast without addressing her gender. This incidental depiction neither affirms nor problematizes trans themes, serving as background representation in a family-oriented tale.

Princess Peach participates in a casino fight where she physically overpowers and defeats several male opponents through hand-to-hand techniques. Rosalina engages in combat but primarily against robotic foes using magical abilities.

The film depicts family through redemptive father-son reconciliation, protective motherhood, and sibling reunions, framing these bonds as enduring and worthy of sacrifice. This positive emphasis on parental roles and relational permanence leans toward traditional family values without significant critique.