The film 'That's My Boy' features a character, Chad, whose effeminate portrayal and perceived sexuality are consistently used as a source of homophobic jokes and ridicule by the protagonist. The narrative presents these harmful stereotypes and mockery without critique, resulting in a problematic and negative overall depiction of LGBTQ+ themes.

The film features a character, Father Tony, who is revealed to be a trans man. However, this revelation is used for shock humor and crude jokes, making the character's trans identity a source of ridicule. The portrayal reinforces negative stereotypes, presenting the transition as bizarre rather than with dignity or respect, resulting in a problematic depiction.

The film portrays Christian characters, particularly Jamie's conservative family and Father McNally, as rigid, judgmental, and hypocritical for comedic effect. The narrative offers no significant counterbalancing positive portrayal or nuance, reinforcing negative stereotypes about conservative religious adherents.