The Ambushers, a 1967 spy film, does not feature any discernible LGBTQ+ characters or themes. The narrative focuses on conventional spy tropes and heterosexual relationships, offering no specific portrayal of queer identity within its content.

The film "The Ambushers" (1967) is a spy thriller centered on espionage and a stolen laser weapon. There are no identifiable transsexual characters or themes present in the narrative. The plot focuses on the protagonist's mission to recover the weapon and investigate a murder, without any elements related to transgender identity.

The film features female characters in spy roles who are involved in action and espionage. However, their victories and contributions to action sequences are primarily achieved through the use of firearms, gadgets, or strategic maneuvering. There are no clear instances of female characters defeating male opponents in direct physical combat.

This film is an adaptation of a novel series. All major characters, including the protagonist Matt Helm, maintain their established gender from the source material. No characters originally established as one gender are portrayed as a different gender in this adaptation.

Research indicates that "The Ambushers" (1967) does not feature any characters who were canonically, historically, or widely established as one race in the source material or prior installments and then portrayed as a different race in this film.