Team America: World Police features a same-sex act played for shock and humiliation, alongside the frequent use of derogatory slurs. These elements contribute to a problematic portrayal where queer identity or same-sex acts are associated with mockery and degradation, lacking any affirming or complex representation.

Team America: World Police includes a transsexual character, Lisa, whose identity is presented as a comedic shock reveal. The film uses her transsexuality as a source of humor and discomfort for other characters, without affording her dignity or complex portrayal, resulting in a problematic depiction.

The film portrays 'terrorists' (visually and rhetorically linked to Islamic extremism) as irrational, violent, and easily manipulated villains. While satirical, the narrative reinforces negative stereotypes without offering counterbalancing nuance or critiquing the prejudice itself.