The Brothers Grimm is a fantasy adventure film focusing on the titular brothers' encounters with magical creatures and events. The narrative does not feature any identifiable LGBTQ+ characters, relationships, or themes, resulting in no direct portrayal or impact on queer identity.

The Brothers Grimm does not feature any identifiable transsexual characters or themes. The film's narrative focuses on a fantastical interpretation of the Grimm fairy tales, with no elements pertaining to transgender identity or experiences.

The film features Angelika, a capable hunter, who is involved in action sequences. However, her combat actions primarily involve defending herself, assisting the male protagonists, or interacting with the environment, rather than directly defeating one or more male opponents in close-quarters physical combat. The Mirror Queen's victories are achieved through magic, not physical combat.

The film features historical figures (the Brothers Grimm) and established fairy tale characters (e.g., Mirror Queen) whose on-screen genders align with their canonical or historical portrayals. No established character undergoes a gender change.

The film portrays the historical figures Jacob and Wilhelm Grimm with actors whose race aligns with their documented historical race. Other significant characters are original to the film or are based on fairy tale archetypes without a previously established race that would constitute a swap.