The Emperor's New Groove does not feature any identifiable LGBTQ+ characters or themes. The narrative focuses on the self-centered Emperor Kuzco's transformation and his relationship with Pacha, without exploring any aspects of queer identity or experience.

The Emperor's New Groove does not feature any identifiable transsexual characters or themes. The plot centers on a magical animal transformation and personal growth, entirely unrelated to gender identity. Therefore, the film has no discernible impact, positive or negative, on the portrayal of transsexual individuals.

The film features female characters like Yzma and Chicha. While Yzma engages in chases and struggles, her victories are not through direct physical combat against male opponents. Chicha uses clever tactics and distractions but does not physically defeat male characters in close-quarters combat.

The Emperor's New Groove is an original animated film, not an adaptation of pre-existing material or historical events. All characters were created for this specific production, establishing their genders within its original narrative. Therefore, no character's gender was altered from a prior canonical or historical depiction.

The film is an original animated story, not an adaptation of pre-existing material with established character races. All characters were created for this film, thus no race swap occurs.