The Flash does not include any identifiable LGBTQ+ characters or themes. The narrative focuses on Barry Allen's personal journey and the multiverse concept without incorporating queer identities or experiences, resulting in no depiction.

The Flash (2023) does not explicitly portray transsexual characters or themes within its narrative. While the film is noted for featuring a non-binary actor in a major role, this aspect of LGBTQ+ representation is external to the movie's storyline and character depictions.

The film features Supergirl, a Kryptonian with superpowers, who engages in combat. Her victories against male opponents, such as prison guards, are achieved through the use of her inherent super strength and other Kryptonian abilities, rather than through skill, martial arts, or non-superpowered physical combat.

The film features Supergirl, who is canonically female and is portrayed as such. No established male or female character from the source material or prior adaptations is depicted with a different gender.