The film features LGBTQ+ coded characters, notably Barbra, whose effeminate and gender non-conforming portrayal as a stalker relies heavily on harmful stereotypes for comedic effect. The net impact is negative due to the depiction of identity as a source of mockery and degradation without counterbalance.

The Ladies Man features characters who cross-dress or are subject to gender-based mistaken identity, primarily for comedic effect. These portrayals often rely on stereotypes and present gender non-conformity as a source of ridicule, contributing to a problematic depiction rather than affirming transsexual themes. The humor is frequently at the expense of the characters' gender presentation.

The film portrays a 'Christian Coalition' group as a censorious and hypocritical antagonist, satirizing their moralistic activism and attempts to shut down Leon Phelps' radio show. The narrative positions the audience to view this specific group's actions negatively, rather than critiquing the broader Christian faith itself.