The film "The Legend of Nigger Charley" does not feature any identifiable LGBTQ+ characters or themes. The narrative focuses on the experiences of an escaped slave in the American West, with no elements related to queer identity or issues present in its plot or character arcs.

The film "The Legend of Nigger Charley" does not feature any identifiable transsexual characters or themes within its narrative. The story focuses on the escape and adventures of an enslaved man in the post-Civil War American South, with no elements related to transgender identity.

The film does not feature any scenes where a female character engages in and wins close-quarters physical combat against one or more male opponents. Female characters are present but are not depicted in such action roles.

The film "The Legend of Nigger Charley" is an original story from 1972, not an adaptation of pre-existing material with established characters. Therefore, no characters were previously established as a different gender in prior canon or history.

The film introduces its original characters, including Charley, as Black. There is no prior source material, historical record, or established canon where these characters were depicted as a different race before this film's production.