The Old Guard features Joe and Nicky, an openly gay immortal couple whose centuries-long relationship is depicted with profound love, dignity, and strength. Their bond is central to their characters and the team dynamic, serving as an affirming portrayal of queer love that is celebrated and respected within the narrative.

The film features multiple female characters, including Andy and Nile, who repeatedly engage in and win close-quarters physical fights against several male opponents. Their victories are achieved through martial skill, strength, and melee weapon use.

Joe's Islamic faith is depicted respectfully, and his relationship with Nicky powerfully illustrates how love and shared humanity can transcend historical religious conflict, promoting understanding and unity.