The Secret of Moonacre does not feature any explicit or implicit LGBTQ+ characters or themes. The story centers on a young girl's adventure in a magical valley, focusing on family history, curses, and a budding heterosexual romance, thus resulting in no portrayal of LGBTQ+ elements.

The film 'The Secret of Moonacre' does not feature any identifiable transsexual characters or themes. The story focuses on a young girl's journey into a magical land, and there are no elements within the plot or character arcs that depict or allude to transgender identity. Therefore, an assessment of portrayal is not applicable.

The film features Maria Merryweather as the protagonist, who resolves conflicts primarily through uncovering secrets, using magical elements, and her wits. No female character is depicted engaging in or winning close-quarters physical combat against male opponents.

The film is an adaptation of the novel "The Little White Horse." A review of the main characters and plot reveals no instances where a character canonically established as one gender in the source material is portrayed as a different gender in the film.

The film adapts "The Little White Horse," set in England with implicitly white characters. All major roles in the 2009 adaptation are portrayed by white actors, aligning with the source material's established racial context.