This animated musical adaptation of 'Tom Sawyer' does not feature any explicit or implicit LGBTQ+ characters or themes. The narrative focuses on the adventures of Tom, Huck, and their friends in a traditional interpretation of the classic story, without incorporating queer identities or storylines.

The animated film "Tom Sawyer" (2000) does not include any transsexual characters or themes. Its narrative focuses on the adventures of Tom Sawyer and his friends, consistent with the source material, without incorporating elements related to transgender identity.

The film is an animated musical adventure based on Mark Twain's novel. Female characters like Becky Thatcher and Aunt Polly are present, but they do not participate in or win any direct physical combat against male opponents.

The 2000 animated film "Tom Sawyer" adapts Mark Twain's novel. All major characters, including Tom Sawyer, Huckleberry Finn, and Aunt Polly, retain their established genders from the source material, despite being portrayed as anthropomorphic animals.

The 2000 animated film "Tom Sawyer" portrays its characters as anthropomorphic animals, such as Tom as a cat and Huck as a fox. This constitutes a species change rather than a race swap, as the characters do not possess human racial identities.