Tour de Pharmacy features characters whose portrayals lean on effeminate stereotypes and implied same-sex relationships for comedic effect. The humor often derives from these elements being presented as scandalous or absurd, reinforcing caricatures rather than offering nuanced or affirming depictions of LGBTQ+ identity. The net impact is negative due to the reliance on problematic stereotypes without counterbalance.

The film depicts JuJu Peppi, a male cyclist who transitions to female solely to exploit a perceived competitive advantage in women's sports. This portrayal frames gender transition as a cynical tactic for cheating, rather than a genuine identity. The narrative uses this premise for mockery, reinforcing harmful stereotypes about trans women in sports and presenting the identity as a source of ridicule.

The film is a mockumentary focused on doping in professional cycling, and its narrative does not contain meaningful content related to family structures, roles, or values, thus offering no clear endorsement or critique of any particular family model.