X-Men: The Last Stand presents a positive allegorical portrayal of LGBTQ+ themes through its central 'mutant cure' storyline. The film champions the right to self-identity and depicts societal pressure to conform as a negative force, with the X-Men fighting to affirm the worth of all identities. This narrative stance provides an empathetic and validating message for marginalized groups.

The film features Mystique, a mutant with enhanced agility and shapeshifting abilities, who effectively defeats multiple male guards in direct physical combat using martial arts and hand-to-hand techniques during a prison transport raid.

The film features several characters, including Callisto, Arclight, and Kid Omega, who were canonically depicted as white in the source comics but are portrayed by actors of different racial backgrounds.