The film "Once Upon a Deadpool" does not feature any identifiable transsexual characters or themes. The narrative and character arcs do not engage with transgender identity in any capacity, resulting in no direct portrayal to evaluate.

While female characters like Domino, Negasonic Teenage Warhead, and Yukio participate in action sequences and engage with male opponents, their victories are primarily achieved through the use of their unique superpowers (luck manipulation, concussive blasts, electricity) rather than direct physical combat skill, strength, or martial arts against multiple male opponents.

This film is a re-release of "Deadpool 2" with added framing scenes. All established characters from the source material or previous installments maintain their canonical gender portrayals.