The film 'Yo-kai Watch Shadowside: The Return of the Oni King' does not include any discernible LGBTQ+ characters or themes. Its narrative focuses on the established universe of Yo-kai and human interactions, without exploring queer identities or relationships.

The film "Yo-kai Watch Shadowside: The Return of the Oni King" does not feature any identifiable transsexual characters or themes. Its narrative focuses on supernatural creatures and adventure, with no elements related to transgender identity or experiences.

Female characters in the film, such as Natsume Amano and Ayame Himeno, primarily participate in combat by summoning powerful Yo-kai to fight on their behalf. They do not engage in or win direct physical confrontations against male opponents using their own martial arts, skill, or strength.

The film introduces new human protagonists and new forms for existing Yo-kai. These new characters are original to the Shadowside storyline, and the established Yo-kai retain their canonical genders despite design changes. No character established as one gender in prior canon is portrayed as a different gender.

This Japanese animated film is part of an established Japanese franchise. All main characters maintain their original racial depictions consistent with the source material, which is Japanese. There are no instances of characters established as one race being portrayed as a different race.