9-1-1: Lone Star offers a predominantly positive portrayal of LGBTQ+ characters. It features a central gay couple whose relationship is depicted with dignity and affirmation, and a transgender character who is a respected team member. The narrative consistently validates queer identities and relationships.

9-1-1: Lone Star features Paul Strickland, a prominent transgender character. His portrayal is consistently positive, depicting him as a competent and respected firefighter/paramedic. The show integrates his trans identity respectfully, focusing on his professional and personal life without reducing him to a stereotype. The narrative affirms his worth and agency within the team.

The show features Marjan Marwani, a firefighter, who is depicted engaging in and winning direct physical altercations against male opponents in several instances, utilizing her strength and combat skills.

The show consistently portrays Christian characters like Grace, Judd, and Tommy as compassionate, moral, and resilient individuals whose faith provides strength, guidance, and a strong community foundation.

Marjan Marwani is depicted as a strong, competent, and respected Muslim firefighter whose faith, including her hijab, is an integral and positive aspect of her identity, actively challenging negative stereotypes.