All Elite Wrestling: Dynamite features openly LGBTQ+ wrestlers like Anthony Bowens and Nyla Rose, who are portrayed with dignity and agency. Their identities are integrated respectfully into their characters and storylines, often celebrated by fans, and not used for mockery or negative stereotypes. This consistent, affirming representation results in a net positive impact.

All Elite Wrestling: Dynamite features Nyla Rose, an openly transsexual woman, as a prominent and successful wrestler, including a former championship reign. Her portrayal emphasizes her athletic prowess and character agency, generally depicting her with dignity. While some in-character antagonisms exist, the show's overall presentation of Nyla Rose is affirming and respectful.

The show features instances where female wrestlers engage in and win direct physical combat against male opponents. This includes a female character achieving a pinfall victory over a male opponent in a wrestling match.