AnoHana: The Flower We Saw That Day centers on themes of grief, friendship, and unrequited love within a group of childhood friends. The narrative does not feature any identifiable LGBTQ+ characters or themes. The story's emotional core revolves around heterosexual relationships and the process of healing from loss.

The film AnoHana: The Flower We Saw That Day does not feature transsexual characters or themes within its narrative. The story centers on a group of childhood friends dealing with the emotional aftermath of a past tragedy and the appearance of a ghost, with no elements related to transsexual identity or experiences present in its plot or character arcs.

The movie does not contain any action or adventure elements.

This original anime series introduces its characters for the first time within its own narrative. There are no pre-existing source materials or prior adaptations where characters' genders were established differently.

The animated series is an original Japanese production. Characters are depicted as their original race, consistent with the production's origin. No character established as one race in prior canon is portrayed as a different race.