"Army Wives" features a lesbian relationship between Gloria Cruz and Penny, introduced after the repeal of "Don't Ask, Don't Tell." Their relationship is portrayed with dignity and emotional complexity, facing challenges common to military couples rather than specific prejudice due to their sexual orientation. The show integrates their story respectfully, contributing to a positive and normalizing depiction of LGBTQ+ individuals in a military setting.

Army Wives features a Season 6 storyline where soldier Kevin comes out as transgender and transitions to Kelli. The portrayal emphasizes the character's authentic journey and the family's emotional adaptation. While depicting external challenges and initial struggles within the military context, the narrative maintains an empathetic and respectful stance towards transgender identity, framing obstacles as external forces.

Christianity is consistently depicted as a significant source of comfort, community, and moral support for many characters, particularly during the challenges of military life. The narrative generally portrays its adherents and practices with respect and understanding.