Barbie: It Takes Two does not feature any explicit LGBTQ+ characters or themes. The narrative centers on friendship, music, and adventure, with no elements that could be interpreted as either affirming or problematic regarding queer identity. Therefore, the net impact is N/A.

The animated series "Barbie: It Takes Two" does not feature any identifiable transsexual characters or themes. The narrative focuses on the adventures of two Barbies pursuing their dreams, with no elements related to transgender identity present in its plot or character arcs.

The movie does not contain any action or adventure elements.

The series features established characters from the Barbie franchise, such as Barbie and Ken, who maintain their canonical genders. No characters previously established as one gender are portrayed as a different gender in this installment.

The show introduces a new, distinct character named Barbie 'Brooklyn' Roberts, who is Black, alongside the original white Barbie 'Malibu' Roberts. This is the creation of a new character, not a race swap of an established one.