"Being Mary Jane" features Patrick Patterson, Mary Jane's gay brother, whose storyline explores his journey of self-acceptance and family dynamics. While initially facing some family resistance, the narrative arc emphasizes growing understanding and support. Patrick is depicted as a complex character, and his story ultimately affirms the worth of his identity and relationships.

Being Mary Jane features a significant storyline involving a transgender character, Nichelle, who becomes a love interest for Mary Jane's brother. The narrative explores themes of acceptance, identity, and prejudice through Patrick's evolving relationship and Mary Jane's journalistic endeavors. The portrayal is generally empathetic, depicting trans characters with dignity and challenging societal biases, leading to a net positive impact.

The show portrays Christianity as a significant and often positive influence in the lives of its characters, providing comfort, community, and a moral framework. While characters may struggle with their faith or exhibit human flaws, the narrative generally respects the religion's role and does not satirize or condemn the faith itself.