Blood Drive includes openly gay characters and explores non-traditional identities within its highly stylized, campy, and satirical grindhouse setting. While LGBTQ+ characters are present and have agency, their portrayals, including some stereotypical elements, are consistent with the show's overall exaggerated tone, resulting in a portrayal that neither strongly uplifts nor denigrates queer identity.

The film satirizes and critiques religious-like institutions and figures, portraying them as corrupt, hypocritical, and manipulative. Characters like the televangelist Julian Slink and the corporate cult of Heart Enterprises, which uses religious rhetoric for control, are depicted as villains, with no counterbalancing positive portrayal of genuine faith.