Brothers & Sisters features a prominent gay character, Kevin Walker, whose relationship and family life are depicted with dignity and complexity. The narrative normalizes LGBTQ+ relationships and families, portraying their challenges as universal human experiences rather than as a consequence of their identity, resulting in a largely affirming portrayal.

The show portrays Christianity primarily as a cultural backdrop and a source of personal comfort or tradition for various characters. While individual characters may struggle with faith or exhibit flaws, the narrative itself treats Christian practices and beliefs with respect, presenting them as meaningful aspects of life for those who adhere to them.

Judaism is depicted positively through the character of Jonathan, Saul's partner, and their wedding. The show respectfully incorporates Jewish traditions, presenting them as an integral and meaningful part of the characters' lives and relationships, without any negative narrative framing.