The film 'Call of the Night' does not feature any explicitly identified LGBTQ+ characters or themes. The central romantic plot is heterosexual, and the narrative does not delve into queer identities or relationships, leading to a determination of N/A for LGBTQ+ portrayal.

Based on available information, 'Call of the Night' does not feature identifiable transsexual characters or themes. The narrative primarily focuses on the relationship between a human boy and a vampire girl, exploring themes of insomnia, freedom, and the unique inhabitants of the night, without incorporating transgender identities into its plot or character arcs.

The movie does not contain any action or adventure elements.

The anime adaptation of "Call of the Night" faithfully portrays its characters with the same genders as established in the original manga series. No major or legacy characters had their gender changed from the source material.

The anime 'Call of the Night' is an adaptation of a Japanese manga. The characters are consistently depicted as East Asian, aligning with their source material. There are no instances where a character canonically established as one race is portrayed as a different race.