Available information for "CITY THE ANIMATION, 2025" indicates no identifiable LGBTQ+ characters or themes. Plot summaries, episode descriptions, trailers, and user reviews consistently lack any mention of queer characters, relationships, or related ideas, suggesting no depiction within the film's narrative.

Based on available information, 'CITY THE ANIMATION' does not appear to feature any identifiable transsexual characters or themes. Plot descriptions, user reviews, and promotional content focus on surreal comedy and everyday life in a unique city, without any mention of gender transformation or related elements. Therefore, there is no depiction of transsexual individuals or themes within the film's known scope.

The movie does not contain any action or adventure elements.

The film's characters maintain their established genders from the source manga. Ayumu Niikura, a male character in the manga, is depicted as male in the animation, despite being voiced by a female actor. This does not constitute a gender swap.

The anime adapts a Japanese manga, features Japanese voice actors, and depicts characters as Japanese university students in a Japanese urban setting, consistent with their source material. There is no indication of any character being portrayed as a different race.