Code Black features a positive portrayal of LGBTQ+ themes through the character arc of Emily Campbell, who comes out as a lesbian. The show depicts her father's initial struggle and eventual acceptance, framing it as a journey of support and validation for queer identity and relationships, contributing to an affirming net impact.

Code Black features a transgender character, Maya, who is a victim of a hate crime. The show portrays her with dignity and agency, while the medical staff treats her with respect and empathy. The narrative frames prejudice as an external force, affirming the worth of transsexual lives and condemning transphobia.

The show often portrays Christian characters, patients, or their families finding comfort, hope, or moral guidance in their faith during medical crises, with the narrative treating these expressions with respect and sympathy.