Da Vinci's Demons does not feature any identifiable transsexual characters or themes. While a male character briefly cross-dresses as a disguise for a mission, this is presented purely as a plot device and not an exploration of gender identity. The series contains no narrative elements that depict or engage with transsexual experiences.

The movie does not contain any action or adventure elements.

The series features numerous historical figures whose on-screen portrayals maintain their documented historical genders. No established characters, whether historical or from prior canon, are depicted with a different gender.

The show primarily features historical European figures who are portrayed by actors matching their historical race. Fictional characters created for the series, such as Zoroaster, do not constitute race swaps as they are original to the show's narrative rather than established historical figures in that specific context.