The 'Dune' universe, as depicted in its major film and television adaptations, does not feature any identifiable LGBTQ+ characters or themes. Consequently, there is no portrayal, positive, negative, or neutral, of LGBTQ+ identities or experiences within the story.

The film "Frank Herbert's Dune" (2000 miniseries) does not feature any identifiable transsexual characters or themes. The narrative focuses on political intrigue, ecology, and human evolution within a science fiction setting, without addressing gender identity or transition.

The 2000 miniseries "Dune" faithfully adapts Frank Herbert's novel, maintaining the established genders for all major and supporting characters. No canonical male or female characters were portrayed as a different gender.

The 2000 miniseries adaptation of Frank Herbert's "Dune" features characters whose on-screen portrayals align with their established racial depictions in the source material. No major or legacy characters were depicted by actors of a different race than canonically established.