Galaxy Express 999 does not feature any identifiable LGBTQ+ characters or themes. The narrative primarily focuses on the journey of Tetsuro Hoshino and Maetel, exploring themes of humanity, mortality, and the future, without incorporating queer identities or relationships into its plot or character arcs.

Galaxy Express 999 does not feature any identifiable transsexual characters or themes. The narrative primarily explores themes of humanity, mortality, and the impact of technology on society through the journey of Tetsuro and Maetel.

The series primarily features Tetsuro and Maetel. While Maetel is a capable character who often protects Tetsuro, her interventions typically involve the use of a firearm or strategic guidance. There are no significant instances where a female character is depicted winning in close-quarters physical combat against one or more male opponents through skill, strength, or martial arts.

The 1978 "Galaxy Express 999" anime is the original adaptation of Leiji Matsumoto's manga. Its main characters, Tetsuro Hoshino and Maetel, retain their established genders from the source material without any changes.

The 1978 animated series "Galaxy Express 999" is an adaptation of the manga. The characters' racial depictions in the anime are consistent with their original portrayals in the source material, with no instances of established characters being depicted as a different race.