Game Grumps features incidental LGBTQ+ references and humor, often normalizing terms and concepts in a lighthearted way. The hosts' general progressive stance ensures that portrayals avoid harmful stereotypes, resulting in a neutral overall impact where queer identity is present but not central to the show's narrative or message.

The show frequently employs humor to highlight perceived absurdities, hypocrisies, or dogmatism within certain Christian contexts, particularly conservative subcultures or specific religious media. This comedic framing often positions adherents or institutions as 'foolish' or 'hypocritical' without significant counterbalancing positive portrayals, aligning with a 'Negative' depiction.

The show frequently features host Dan Avidan sharing personal anecdotes, cultural references, and experiences related to his Jewish heritage. These portrayals are consistently presented with warmth, humor, and respect for his personal identity, often highlighting cultural aspects rather than religious dogma, and never framing Judaism itself in a problematic light.