Gen V portrays LGBTQ+ characters and themes in a normalized, natural, and positive manner. Characters like Jordan, a bigender shapeshifter, and Marie, in a same-sex relationship, explore their identities authentically. The show avoids making queerness a source of shame or conflict, presenting fully realized individuals whose identities are accepted by peers, emphasizing queer joy and everyday existence.

Gen V portrays Jordan Li, a bigender shapeshifting superhero, with authenticity and complexity. The series explores Jordan's identity, challenges with parental acceptance, and societal pressures, using their powers as a metaphor for gender fluidity. The narrative integrates these themes respectfully, depicting queer relationships as normalized and affirming the worth of trans and gender-nonconforming lives within its superhero context.

The show features Ant Woman, who engages in and wins a close-quarters physical fight against a male guard. Other female characters utilize superpowers or engage in inconclusive physical struggles against male opponents.