Go, Diego, Go! is a children's animated educational series for preschoolers, primarily focused on animal rescue and environmental themes. The show's content and narrative scope do not feature any identifiable LGBTQ+ characters or storylines, leading to a classification of N/A for LGBTQ+ portrayal.

The children's animated series 'Go, Diego, Go!' does not include any identifiable transsexual characters or themes. Its narrative is centered on a young boy, Diego, who helps animals and teaches about the natural world, without engaging with topics of gender identity.

The animated children's show 'Go, Diego, Go!' focuses on animal rescue and environmental education. Female characters like Alicia and Dora primarily use problem-solving and knowledge to overcome challenges, rather than engaging in direct physical combat with any opponents.

Go, Diego, Go! is an original animated series and a spin-off of Dora the Explorer. Its main characters, Diego and Alicia, were created for the show and do not have prior canonical gender identities that were altered. Returning characters like Dora maintain their established gender.

The characters in "Go, Diego, Go!" were created as Latino for the show and its parent series, "Dora the Explorer." Their on-screen portrayal is consistent with their original design, with no instances of characters being depicted as a different race than their established canon.