Hazbin Hotel offers a progressive and inclusive portrayal of LGBTQ+ characters, normalizing diverse sexual orientations and gender identities within its narrative. Relationships like Charlie and Vaggie's are presented as natural, and the show avoids stereotypes, treating queerness as an accepted part of its universe without homophobia or transphobia. This approach humanizes and integrates LGBTQ+ experiences into the show's core themes.

Hazbin Hotel subtly portrays transsexual characters and themes through visual cues and character design, rather than explicit dialogue. The show's universe emphasizes acceptance, pride in identity, and the ability for characters to express their gender through physical redesign. This implicit integration into world-building creates a supportive and affirming representation of trans experiences within its fantastical setting.

The film critiques traditional Christian concepts by depicting Heaven and its leadership as hypocritical, rigid, and unforgiving. This satirical portrayal contrasts with the show's themes of redemption, reinforcing a negative view of these specific religious institutions and their interpretations of morality.