Insatiable features multiple LGBTQ+ characters, most notably Bob Armstrong's journey of self-discovery and Bob Barnard's openly gay antagonist role. While the show's satirical and often crude humor can lead to problematic depictions, it also includes elements of self-acceptance and normalized queer identities. The net impact is neutral, as positive representation is balanced by the show's messy, comedic framing rather than a clear affirming or denigrating stance.

The show features Dixie Sinclair, a prominent transsexual character whose identity is revealed as a plot twist and often tied to her antagonistic role. While she possesses agency, her portrayal frequently associates her trans identity with secrets and manipulation, contributing to a problematic narrative rather than an affirming one.

The series frequently uses Christian characters, particularly Bob Armstrong, to satirize hypocrisy, judgmentalism, and the performative aspects of faith within Southern American culture and beauty pageants. The narrative consistently highlights the disconnect between stated Christian values and the characters' often morally ambiguous actions. There are no significant counterbalancing positive portrayals of the religion itself.