Knightfall features a prominent same-sex relationship between Gawain and De Nogaret in its second season. This relationship is integral to the characters' arcs, marked by manipulation and tragedy, reflecting the show's overall dark tone. The portrayal neither explicitly affirms nor denigrates their queer identity, treating it as a complex personal connection within a morally ambiguous narrative.

The show consistently highlights the corruption, hypocrisy, and political manipulation within the Catholic Church, the Papacy, and some Templar factions. While individual characters may display genuine faith, the overarching narrative critiques the institution's actions and the violence committed in its name.

The film portrays Muslim characters, particularly leaders like Saladin, with respect, strategic intelligence, and a strong sense of their own faith and honor. While they are adversaries to the Templars, the narrative avoids demonization, presenting them as complex and dignified.

The film portrays Jewish characters and communities as victims of Christian persecution, often highlighting their resilience and dignity in the face of injustice. The narrative condemns the bigotry and violence directed towards them by Christian authorities.