The show acknowledges Loki's bisexuality and gender fluidity as inherent aspects of his character through casual dialogue and a character file. These elements are presented matter-of-factly without being central to the plot or a source of conflict, leading to a neutral overall impact.

The show acknowledges Loki's gender fluidity through a detail on their TVA file, listing 'Sex: Fluid.' This inclusion is a factual nod to the character's comic and mythological background, but it is not explored as a central theme or character arc within the narrative, resulting in an incidental portrayal.

The show features Sylvie, who repeatedly defeats male TVA agents in physical combat using a sword and martial arts. Ravonna Renslayer also physically overcomes a male opponent in close quarters.

The character Loki, canonically established as male in source material and prior adaptations, is portrayed with a prominent female variant, Sylvie Laufeydottir, who is explicitly a version of Loki. This constitutes a gender swap.

The show features race swaps for established Marvel characters. Ravonna Renslayer, traditionally depicted as white in the comics, is portrayed by a Black actress. Additionally, He Who Remains, a variant of the canonically white Kang the Conqueror, is played by a Black actor.