Lucifer features prominent bisexual/pansexual characters like Maze and Eve, whose romantic relationship is depicted with dignity and complexity. Their love story is a significant, positive arc, facing typical relationship challenges rather than prejudice. The series normalizes diverse sexual orientations, contributing to an affirming portrayal of LGBTQ+ identities.

The show "Lucifer" adapts characters from DC Comics and religious mythology. Several angels, including Azrael, Remiel, and Gabriel, who are canonically or widely established as male in source material, are portrayed as female in the series, constituting gender swaps.

Amenadiel, a character depicted as white in the source comics (The Sandman), is portrayed by a Black actor in the show. Mazikeen, also depicted with a non-Black appearance in the comics, is portrayed by a mixed-race actress.

The show uses Christian mythology as its foundation, exploring themes of free will, redemption, and the nature of good and evil with depth and nuance. While it critiques rigid dogma and human misinterpretations, it ultimately portrays the divine (God, angels) in a complex but often benevolent light, affirming spiritual virtues like love, sacrifice, and justice.