Marco Polo features Jingim, Kublai Khan's son, in a discreet romantic relationship with his male attendant. This portrayal is respectful, adding depth to Jingim's character without being central to the plot or a source of mockery. The narrative handles their connection with dignity, leading to a neutral overall impact regarding LGBTQ+ themes.

The show features female characters Khutulun and Mei Lin. Khutulun, a warrior, is shown defeating male opponents in wrestling and melee combat. Mei Lin, an assassin, repeatedly engages in and wins close-quarters physical fights against multiple male guards and soldiers.

Byamba, a historical Mongol general, is portrayed by a Tongan actor. As Mongol (East Asian) and Tongan (Pacific Islander) represent distinct broad racial categories, this constitutes a race swap.

Buddhism is consistently portrayed with profound respect, wisdom, and philosophical depth, often serving as a moral and spiritual guide through characters and teachings.

The show portrays Marco Polo's Christian background with respect, contrasting it with other faiths without demonizing it. It highlights the diversity of Christian expression within the Mongol Empire.

Islam is depicted as a respected and integrated faith within the diverse Mongol Empire, with Muslim characters contributing significantly without negative stereotyping.