Mayfair Witches intentionally prioritizes and normalizes LGBTQ+ representation, weaving diverse queer characters and themes into the show's core identity. The creators aimed for inclusivity, making queer experiences central to the narrative and atmosphere, affirming the worth of LGBTQ+ lives within its supernatural setting.

Mayfair Witches features Jojo, a trans woman, depicted as an empowered and respected Mayfair witch. Her trans identity is naturally integrated into the narrative, treated without comment as equal to other women witches, and not a source of conflict. The portrayal emphasizes dignity, equality, and acceptance, contributing to a normalizing and affirming representation within the series' diverse cast.

The series portrays Christianity with tension, highlighting fanatical beliefs through characters like Carlotta and depicting its institutions as challenged by supernatural forces. Historical Christian conflicts also contribute to a narrative that uses the faith as a backdrop for struggle rather than affirmation.