There is not enough information available to determine the presence or portrayal of transsexual characters or themes in 'Midnight Diner: Tokyo Stories, 2016'. Without plot details or character arcs, a specific assessment of positive, negative, or neutral impact cannot be made.

The movie does not contain any action or adventure elements.

Midnight Diner: Tokyo Stories is an adaptation of a manga series. There is no evidence that any character canonically established as one gender in the source material was portrayed as a different gender in this series. The show primarily introduces new characters or faithfully adapts existing ones.

Midnight Diner: Tokyo Stories is a Japanese series based on a Japanese manga, featuring an entirely Japanese cast portraying Japanese characters in a Japanese setting. There is no evidence of any character being established as one race in prior canon and then portrayed as a different race in this adaptation.